(P23-061-23) Chemicalisotope-Labelling LC/MS and HPLC Analysis of Coffee Containing Javamide-I/-II and In Vivo Effects on Metabolic/Inflammatory Factors in Rats Fed a High Fat Diet
Principal Investigator BHNRC, ARS, USDA Beltsville, Maryland, United States
Disclosure(s):
Jae Park, PhD: No relevant financial relationship(s) with ineligible companies to disclose.
Objectives: Previously, we reported in vivo effects of coffee containing javamide-I/-II (CCJ12) on metabolic/inflammatory factors in rats fed a normal diet. However, there is still limited information about the effects of CCJ12 on bodyweight and other metabolic factors in the obese. Therefore, in vivo effects of CCJ12 on metabolic/inflammatory factors were investigated in an obesity rodent model.
Methods: Chemical isotope-labelling LC/MS (CIL-LC/MS) and HPLC methods were used to analyze CCJ12. For an animal study, rats were divided into three groups: NG group (a normal diet with drinking water (n=10)), FG group (a high fat diet with drinking water (n=10)), and FCG group (a high fat diet with drinking water containing CCJ12 (n=10)), and the study was conducted for 20 weeks. Food/water consumption and body weight of rats were monitored weekly. Also, metabolic/inflammatory factors (LDL, HDL, total cholesterol, adiponectin, C-reactive protein, sE-selectin, TNF-alpha and MCP-1) were determined in the plasma samples from animals.
Results: CCJ12 was found to contain javamide-I (0.2 mg), javamide-II (1.49 mg), chlorogenic acids (56.8 mg) and caffeine (89.6 mg) per 250ml by HPLC. Also, more than 900 compounds were detected in CCJ12 by CIL-LC/MS. During the study, there was no significant difference in water/food intake between the three groups. However, a significant weight gain was observed in the FG and FCG groups, compared to the NG group (P < 0.05), but there was no significant difference in plasma LDL, HDL, and total cholesterol levels in between the FG and FCG groups. Likewise, there was no significant difference in adiponectin, C-reactive protein, and sE-selectin levels between the FG and FCG groups, suggesting that CCJ12 may have no adverse effects on these factors in the FCG group, compared to the FG group. However, the levels of TNF-alpha and MCP-1 were surprisingly lower in the FCG group than the FG group, indicating that CCJ12 may have some positive effects on the cytokines in the group.
Conclusions: CCJ12 may have no adverse effects on bodyweight, LDL, HDL, total cholesterol, adiponectin, leptin, C-reactive protein, and sE-selectin, rather positive effects on TNF-alpha, and MCP-1 in the rat fed a high fat diet.
.jpg "Jae Park, PhD photo")