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Topical Area: Food Science and Nutrition

Food Science and Nutrition (Poster Session)

(P06-051-23) Nutritional Control Alters the Metabolome of a Novel Probiotic Akkermansia Muciniphila

Saturday, July 22, 2023
12:00 PM – 1:00 PM ET
Location: Poster Board 48

    Presenting Author(s)

  • HZ

    Huan Zhang, PhD

    Postdoctoral Associate
    The Ohio State University
    Columbus, Ohio, United States

    • Co-Author(s)

  • JZ

    Jiangjiang Zhu, PhD

    Assistant Professor
    The Ohio State University
    Columbus, Ohio, United States

  • RX

    Rui Xu

    The Ohio State University

  • SZ

    Shiqi Zhang

    The Ohio State University

  • LC

    Li Chen

    The Ohio State University

Disclosure(s):
Huan Zhang, PhD: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: The gut microbiome is considered as one of the contributing factors to host health and diseases. Akkermansia muciniphila (A. muciniphila) has been identified as a novel probiotic in the last two decades, due to its beneficial effects on a variety of human health conditions, likely due to its metabolic contributions. However, the comprehensive metabolome for A. muciniphila under nutritional control has not been fully mapped to date. Therefore, the present study aims to elucidate the metabolome of A. muciniphila in response to different nutritional perturbations in vitro, so that we can establish foundations to understand its subsequent implications on host diseases and health.

Methods: In the present study, a high-resolution mass spectrometry (HRMS) coupled with high-performance chromatography (HPLC) were utilized to unveil the metabolic changes of A. muciniphila resulting from supplementing four major nutrients in the human gastrointestinal tract: mucin, bile salts, short-chain fatty acids, and inorganic salts in fourteen matrix combinations.

Results: Our preliminary results showed that feeding four major nutrients results in significantly different metabolomes of A. muciniphila. For example, mucin was shown to drive the metabolic differences of A. muciniphila and SCFAs were observed to inhibit the growth of A. muciniphila, thus the production of nucleotide-related metabolites under this nutrient condition were lowered considerably. However, feeding bile salts promotes A. muciniphila growth and produces more secondary bile acids for the host to access. SCFAs in contrast were found to have less impact to the metabolome changes of A. muciniphila in our study.

Conclusions: Nutritional control will significantly change the metabolome of A. muciniphila and thus potentially influence the beneficial outcomes to host health and diseases, particularly in the proliferation of A. muciniphila and the bile acid metabolism

Funding Sources: National Institute of General Medical Sciences