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Session: Meal Timing and Diet Quality for Prevention of Obesity and/or Cardiometabolic Disease (Oral 14)

(OR14-03-23) Time-Restricted Feeding Reduces Body Weight and Fat Mass via Altering Meal Patterns and Energy Expenditure in Old Female Mice Fed High Fat Diet

Monday, July 24, 2023
8:24 AM – 8:36 AM ET
Location:  Constitution B
ELPC

    Presenting Author(s)

  • Theresa M. Bushman, BS

    PhD Candidate
    University of Minnesota
    Hugo, Minnesota, United States

    Disclosure(s): No relevant financial relationship(s) with ineligible companies to disclose.

    • Co-Author(s)

  • HS

    Hongming Su

    University of Minnesota

  • XC

    Xiaoli Chen, MD, PhD

    Professor
    University of Minnesota
    St. Paul, Minnesota, United States

Disclosure(s):
Theresa M. Bushman, BS: No relevant financial relationship(s) with ineligible companies to disclose.
Objectives: The objective of this study was to determine whether time-restricted feeding (TRF) could mitigate the negative effect of a high-fat diet (HFD) on energy balance in old female mice.

Methods: Female mice from 17 to 21 months of age were fed a control diet or a HFD for 12 weeks. The HFD group was then divided into two subgroups: one group was subjected to TRF (food access for 10 hours each day) for ten weeks, while the other group had access to food ad libitum. Body weight, body composition, food intake/meal patterns, glucose and insulin tolerance tests, and indirect calorimetry measurements were conducted.

Results: Mice on the HFD had significantly higher body weight and fat mass than the control mice. TRF significantly decreased body weight and fat mass compared to HFD ad libitum even though total food consumption was not different between the groups. Lean mass did not change significantly between the groups. Interestingly, the TRF group had significantly reduced meal frequency and meal duration but an increased trend in meal size compared to the control group. Glucose and insulin tolerance tests showed a decrease in glucose tolerance and insulin sensitivity in the HFD groups compared to the control. TRF was unable to effectively reverse this detrimental effect of HFD. However, TRF could significantly reduce the HFD-induced elevation of fasting blood glucose levels in the HFD group. While both VO2 and VCO2 were significantly reduced, heat production was increased in the HFD group compared to the control group. The VO2 and VCO2 measurements showed an upward trend back toward the control level for the TRF mice. Heat production trended downward towards the control level in the TRF group. Lastly, physical activity was decreased within the HFD group compared to the control. TRF did increase activity but not significantly compared to HFD.

Conclusions: Overall, our results suggest TRF may have a beneficial impact on body weight and composition, as well as energy intake and expenditures such as meal patterns, VO2, VCO2, heat expenditure, and physical activity. Therefore, TRF may potentially reverse HFD-induced changes seen in obesity in old female mice when combined with a high-fat diet.

Funding Sources: Research supported by Healthy Foods Healthy Lives Graduate Student Research Grant from Healthy Foods, Healthy Lives Institute at the University of Minnesota and NIDDK Grant (R01 DK123042).