(P03-077-23) Palmitoleic Acid Ameliorates Metabolic Disorders and Inflammation by Modulating Gut Microbiota and Serum Metabolites

Objectives: To investigate the regulation of omega-7 palmitoleic acid (POA) on lipid and glucose metabolism, as well as systemic inflammation in high fat dirt (HFD)-fed mice from the perspective of serum metabolome and gut microbiome

Methods: Thirty-six C57BL/6 male mice were randomly assigned to either a normal chow diet containing 1.9% (w/w) lard or an HFD containing 20.68% (w/w) lard or 20.68% (w/w) sea buckthorn pulp oil for 16 weeks.

Results: We found that POA significantly attenuated hyperlipidemia, insulin resistance, and inflammation in HFD-fed mice. POA supplementation significantly altered the composition of serum metabolites, particularly lipid metabolites in the glycerophospholipid metabolism pathway. POA reshaped the composition of gut microbiota, obviously increased the abundance of Bifidobacterium and decreased the abundance of Allobaculum. Importantly, we found that glycerophosphocholine mediated the effect of Bifidobacterium on LDL-C, sphingomyelin mediated the effect of Bifidobacterium on IL-6, and maslinic acid mediated the effect of Allobaculum on IL-6.

Conclusions: Our results suggest that exogenous POA could improve metabolic disorders and inflammation in HFD-fed mice, potentially by modulating the serum metabolome and gut microbiome.

Funding Sources: This work was financially supported by “The National Natural Science Foundation of China” (grant numbers 82103828, 32072224, 32001693, and 32172214).