Yoon Sin Oh, PhD: No relevant financial relationship(s) with ineligible companies to disclose.
Objectives: Allomyrina dichotoma larvae (ADL) is an edible insect with various biological activities such as anti-diabetic, anti-hepatofibrotic and anti-oxidant effects, but its usefulness as an anti-aging ingredient has not been widely studied.
Methods: Thus, this study aimed to evaluate the anti-photoaging effects of ADL extracts (ADLE) and investigated the underlying mechanisms of ultraviolet B (UVB)-treated human diploid fibroblast (HDF) cells,
Results: Among the various solvent fractions, the ethyl acetate (EA) fraction revealed high polyphenol content and high antioxidant activity. UVB-treated HDF cells generated intracellular reactive oxygen species and DNA damage, but the level was attenuated by ADLE–EA fraction-treated cells. The expression levels of aging markers, such as p16INK4a, p21WAF1, and SA-b-gal, increased in UVB-treated cells, and it was considerably reduced by ADLE–EA fraction cotreatment. Moreover, increased matrix metalloproteinase (MMP)-1 and nuclear factor-κB (NF-κB) activation as well as the degradation of COL1A1 by UBV-induced aged cells were reversed by ADLE–EA fraction treatment.
Conclusions: In conclusion, ADLE has an anti-aging effect by inhibiting extracellular matrix degradation levels, and this effect was mediated by NF-κB signaling pathway.
Funding Sources: This research was funded by the basic science research program grant provided by the National Research Foundation of Korea (NRF), which is funded by the Ministry of Science, ICT and Future Planning.
