(P20-044-23) The Relationship Between Gut Microbiota and Nutrients in Korean Adults With Irritable Bowel Syndrome (IBS)

Objectives: It is well known that dietary intake has a significant relationship with the gut microbiota environment of patients with irritable bowel syndrome (IBS). Therefore, this study aimed to analyze the relationship between gut microbiota and nutrients in Korean adults aged 20-39 with IBS.

Methods: This study was conducted on 52 participants (male: 17, female: 35) aged 20-39 years who consented to the study and were selected based on ROME IV criteria. The survey items included general information, dietary intake survey data, and gut microbiota data. The gut microbiota was analyzed by amplifying the 16S ribosomal RNA gene and profiling the microbial community composition (Theragen Bio, Gyeonggi-do, Republic of Korea). Gut microbiota analysis was conducted by calculating the relative abundance of gut microbiota based on taxonomy OTUs data. Statistical analysis was performed using generalized linear regression analysis with gender, age, and total energy intake adjusted for analyzing the relationship between gut microbiota and nutrients. All statistical analyses were performed using R with a significance level of less than 0.05.

Results: The mean age of the subjects was 26.4±5.3 years and their energy intake was 1784.8±675.6 ㎉/day. The analysis of the relationship between nutrients and gut microbiota showed that at the phylum level, Bacteroidota had significant correlations with carbohydrates (β=-0.116, p=0.003) and fats (β=0.295, p=0.007). At the genus level, Bacteroides had a significant correlation with soluble dietary fiber (β=4.525, p=0.032), Blautia had a significant correlation with excess fructose (β=4.525, p=0.05), Agathobacter had a significant correlation with protein (β=-0.043, p=0.0135), Collinsella had a significant correlation with lactose (β=-0.087, p=0.040), and Anaerostipes had a significant correlation with carbohydrates (β=-0.087, p=0.040).

Conclusions: This study revealed that the gut microbiota in patients with IBS is associated with different nutrients. Therefore, understanding the interaction between nutritional components and gut microbiota during meals may be a factor in creating a healthy gut environment for patients with IBS.

Funding Sources: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIP) (No.NRF-2020R1A2C1014177).