Undergraduate Researcher The Ohio State University New Albany, Ohio, United States
Disclosure(s):
Shanvanth Rayal Arnipalli, BS: No relevant financial relationship(s) with ineligible companies to disclose.
Objectives: AACs will induce cytotoxicity and oxidative stress to decrease MM cell viability via disruption of BCMA pathway. BCMA is the specific target for this study because it is responsible for survival, cancer cell growth, drug resistance in MM (Iannazzo et al., 2020), and is a key target of interest in novel immune therapies for MM.
Methods: In this project we aim to test nine novel structurally different amino acid compounds (AAC) for their cytotoxicity and effect on B-cell maturation antigen (BCMA) levels in multiple myeloma (MM) cell lines in their bone marrow environment in vitro for the first time. AACs are small, charged compounds with modified side chains that can self-assemble into nanostructures (Lee et al., 2020). MM is a malignancy of plasma cells residing in bone marrow (BM), and current treatments for MM are very expensive and toxic due to off target effects in BM microenvironment (BMM). This issue provokes the necessity to develop a robust drug to effectively interact with MM cells, which potentially increases the BMM residual time. To address this biomedical need, we propose to use innovative nanomaterials, such as AACs for the investigation of their effects on MM. Although AACs have not been studied before in the context of cancers, including MM; however, their efficacious properties were demonstrated in treating obesity-related conditions (Lee et al., 2020). These observations are relevant because obesity is a critical risk factor for MM incidence, and da Cunha Júnior et al. (2021) have demonstrated that obesity and MM share etiopathogenic mechanisms. Moreover, all key proteins implicated in pathogenesis of MM, such as BCMA, are also expressed in adipose tissue. AAC properties influencing adipocyte biology could also affect MM cells, and thus, the uniqueness of this study stems from investigation of the impact of AACs on both B-cell biology and MM cytotoxicity. BCMA is the specific target for this study because it is responsible for survival, cancer cell growth, and drug resistance in MM cells (Iannazzo). Thus, AACs could be ideal candidates for experimentation.
Results: This abstract contains protocol study methods.
Conclusions: This abstract contains protocol study methods.
Funding Sources: American Society for Nutrition Foundation Undergraduate Diversity Research Fellowship
