(P31-007-23) Diet Composition Influences the Effect of High Fat Diets on Bone in Growing Male Mice

Objectives: The effect of diet-induced obesity on bone in rodents is variable, with bone mass increases, decreases, and no impact reported. The goal of this study was to evaluate whether the composition of obesogenic diet may influence bone independent of its effect on body weight. As proof-of-principle, we used a mouse model to compare the skeletal effects of a commonly used high fat ‘Western’ diet and a modified high fat diet.

Methods: The modified high fat diet included powdered walnut and was isocaloric for macronutrients, but differed in fatty acid composition and contained nutrients (e.g. polyphenols) not present in the standard ‘Western’ diet. Eight-week-old mice (n = 8/group) were randomized into: (1) low fat control diet (LF; 10% kcal fat); (2) high fat ‘Western’ diet (HF; 46% kcal fat as soybean oil and lard); or (3) modified high fat diet supplemented with ground walnuts (HF + walnut; 46% kcal fat as soybean oil, lard, and walnut) and maintained on their respective diets for 9 weeks. Bone response in femur was then evaluated using dual energy x-ray absorptiometry, microcomputed tomography, and histomorphometry.

Results: Consumption of both obesogenic diets resulted in increased weight gain but differed in impact on bone and bone marrow adiposity in distal femur metaphysis. Mice consuming the high fat ‘Western’ diet exhibited a tendency for lower cancellous bone volume fraction and connectivity density, and had lower osteoblast-lined bone perimeter and higher bone marrow adiposity than low fat controls. Mice fed the modified high fat diet did not differ from mice fed low fat diet in cancellous bone microarchitecture, or osteoblast-lined bone perimeter, and exhibited lower bone marrow adiposity compared to mice fed the ‘Western’ diet.

Conclusions: This study demonstrates that two obesogenic diets, similar in macronutrient distribution and induction of weight gain, can have different effects on cancellous bone in distal femur metaphysis. Because the composition of the diets used to induce obesity in rodents does not recapitulate a common human diet, our finding challenges the translatability of rodent studies evaluating the impact of a high fat diet on bone.

Funding Sources: The National Institute of Food and Agriculture, Agricultural Experimental Station Multi-state, Oregon Agricultural Experiment Station