(P25-006-23) Sex Differences in Indicators of Cellular Inflammation In Mice With Sucrose-Induced Nonalcoholic Fatty Liver

Objectives: To evaluate the effect of a high sucrose diet on the expression of the adiponectin gene (Adipoq) and inflammation markers in the progression of non-alcoholic fatty liver disease in male and female mice of the C57BL/6N strain.

Hypothesis:
1. A high sucrose diet decreases the expression of the adiponectin gene and increases the expression of inflammatory markers in male and female mice of the C57BL/6N strain.
2. There is a sex-dependent association between alterations in Adipoq gene expression and inflammation markers in male and female C57BL/6N mice induced with a high-sucrose diet.

Methods: The liver and adipose tissue of mice that were induced in a previous study with non-alcoholic fatty liver with a 50% sucrose solution were studied. The mice were divided into four groups distributed as follows: control females, sucrose females, control males, sucrose males. RNA extraction was performed from liver tissue and adipose tissue samples with the trizol reagent insert. Subsequently, the samples were reverse transcribed with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to obtain cDNA. Real-time PCR was performed for the Adipoq genes in adipose tissue tnf, Il-1𝑏, and Il-6 in liver tissue. The expression of the β-actin gene will be used as the constitutive gene. The relative expression of the genes of interest with respect to the housekeeping gene was calculated using the 2-△△cq quantification method.

Results: Expression of the adipoq gene was decreased in male and female mice on a high-sucrose diet compared to the control group. In contrast, the expression of tnf, Il-1b, and Il-6 was increased in male and female mice on a high-sucrose diet compared to the control group.

Conclusions: A diet high in sucrose contributes to the development of non-alcoholic fatty liver in male and female mice of the C57BL6/N strain, observing a sex-dependent association between alterations in Adipoq gene expression and inflammation markers.

Funding Sources: National Council for Science and Technology in Mexico