(P30-020-23) Anti-Inflammatory Effects of a Maple Syrup Extract on Mice Peritonitis Characterized by A Proteomic Analysis of Kidney

Objectives: Maple syrup extract (MSX), a phenolic-enriched food extract derived from maple syrup, exerted anti-inflammatory by suppressing the production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-ɑ, interleukin (IL)-6, IL-1β in kidney tissues in a lipopolysaccharide (LPS)-induced mouse peritonitis model. However, molecular targets and pathways of MSX’s anti-inflammatory effects are still unclear.

Methods: Herein, MSX’s anti-inflammatory effects were explored by using data-independent acquisition (DIA) proteomics performed on the kidney samples from mice in the model group (challenged with LPS; 1 mg/kg; via intraperitoneal injection; model group), MSX treatment group (60 mg/kg; orally administered for ten days before exposed to LPS), and control group (orally administered phosphate-buffered saline). The kidney proteins were extracted with chloroform-methanol for a sequential window acquisition of all theoretical mass spectra (SWATH)-DIA acquisition on a microflow liquid chromatography with TripleTOF mass spectrometry.

Results: The proteomic analyses of a total 3136 proteins led to the identification of 152 proteins with significant changes between the LPS-induction group and the control group as well as 326 proteins with significant changes between the MSX-treated group and the model group. Four proteins, namely, C-X-C motif chemokine 10 (Cxcl10), solute carrier family 22 member 7 (Slc22a7), mitochondrial import receptor subunit TOM7 homolog (Tomm7), and acylcarnitine hydrolase (Ces2c), were upregulated by 672.01-, 2,33-, 1.94-, and 1.23-fold, respectively, in the LPS-exposed group as compared to the control group, which were decreased by the treatment with MSX by 76.7%, 79.8%, 74.0%, and 79.3%, respectively. Furthermore, ingenuity pathway analysis (IPA) revealed that several upstream regulators including interferon-gamma, IL-1β, TNF, and amyloid beta precursor protein were triggered by the MSX treatment as compared to the model group.

Conclusions: Our findings provided insights into MSX’s modulatory effects on cytokine release and inflammation signaling pathways, which are useful for understanding the anti-inflammatory effects of MSX.

Funding Sources: This project was supported by the Quebec Maple Syrup Producers (Quebec, Canada).