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Topical Area: Obesity

Obesity (Poster Session)

(P31-029-23) The Impact of the Expression Level of Prohibitin 1 on the Cholesterol Metabolism

Monday, July 24, 2023
12:45 PM – 1:45 PM ET
Location: Poster Board 251
Disclosure(s):
Soohan Jung, Ph.D student: No relevant financial relationship(s) with ineligible companies to disclose.


Objectives: Prohibitin 1 (Phb1) is a protein that related in liver function and antioxidant mechanism. In the previous study, we conducted RNA sequencing analysis in liver which differentially expressed Phb1. From that result, we found lipid and cholesterol related genes are down regulated. Based on these results, we hypothesized the role of Phb1 as a key regulator in cholesterol metabolism.

Methods: In order to control prohibitin1 expression level, we used HepG2 cell line, phb1 expression was reduced using siPhb1, and phb1 was overexpressed using Phb1 overexpression vector. After that, we treated 0.2mg/mL of cholesterol in media for 24 hours.

Results: In the Phb1 knock-down group, the mRNA expression level of HMG-CoA reductase and Srebp2 were significantly increased. After cholesterol treatment, mRNA expression levels of those genes were reduced in control groups. However, Phb1 KD groups still expressed those genes. In the Phb1 over-expressed group showed no differences compared to control group.

Conclusions: Through these results, it was found that when the expression of Phb1 was reduced, cells have problems with cholesterol homeostasis control. In particular, it was confirmed that Phb1 affects cholesterol metabolism through the regulation of the expression of Srebp2, a key regulator important for cholesterol homeostasis. Taken together, these results suggest that maintenance of cholesterol homeostasis in the liver through regulation of the expression level of Phb1 in the liver may be beneficial to liver health.

Funding Sources: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government Ministry of Science and ICT.